Pep Guardiola makes seven changes to the Manchester City side that beat Manchester United 3-1 on Sunday.
Rico Lewis, Oscar Bobb and Matheus Nunes are among the starters, with Kevin de Bruyne, Bernardo Silva and Phil Foden among the substitutes.
Manchester City: Ederson, Lewis, Akanji, Dias, Gvardiol, Rodri, Kovacic, Bobb, Alvarez, Nunes, Haaland.
Subs: Ortega, Carson, Walker, Stones, Ake, De Bruyne, Silva, Gomez, Foden, Wright, Susoho, Hamilton.
FC Copenhagen are third in the Danish Superliga and were replaced at the top by Midtjylland, who they lost 2-0 to at the weekend.
Scotland international defender Scott McKenna, on loan from Nottingham Forest, starts, while Oscar Hojlund, the younger brother of Manchester United striker Rasmus, is on the bench.
FC Copenhagen XI: Grabara, Diks, McKenna, Vavro, Jelert, Froholdt, Oskarsson, Ankersen, Clem, Achouri, Elyounoussi.
Subs: Runarsson, Buur, Sorensen, Boilesen, Mattson, Bardghji, Meling, Larsson, Cornelius, Hojlund.